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pmscv loxp  (Addgene inc)


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    Structured Review

    Addgene inc pmscv loxp
    Pmscv Loxp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmscv+loxp/pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE+(Plasmid+%2332702)/pmc12766950-451-7-8
    Average 93 stars, based on 39 article reviews
    pmscv loxp - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development.
    Article Snippet: The PCR product from Gibson assembly was purified and cloned into pJet1.2 plasmid (CloneJET PCR Cloning Kit, Thermo Fisher Scientific). .. Then, the loxP-dsRed-loxP fragment was cloned from pMSCV-loxP-dsRed-loxP-3xHA-Puro-WPRE (Addgene plasmid #32703). .. The loxP-dsRed-loxP fragment and the above modified pJet1.2 plasmid were digested with restriction enzymes AgeI (NEB, R0552S) and PacI (NEB, R0547S) and then ligated using T4 DNA ligase (NEB, M0202S) to get pAW63-Tex10 plasmid.

    Article Title: Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development
    Article Snippet: The PCR product from Gibson assembly was purified and cloned into pJet1.2 plasmid (CloneJET PCR Cloning Kit, Thermo Fisher Scientific). .. Then, the loxP-dsRed-loxP fragment was cloned from pMSCV-loxP-dsRed-loxP-3xHA-Puro-WPRE (Addgene plasmid #32703). .. The loxP-dsRed-loxP fragment and the above modified pJet1.2 plasmid were digested with restriction enzymes AgeI (NEB, R0552S) and PacI (NEB, R0547S) and then ligated using T4 DNA ligase (NEB, M0202S) to get pAW63-Tex10 plasmid.

    Plasmid Preparation:

    Article Title: Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development.
    Article Snippet: The PCR product from Gibson assembly was purified and cloned into pJet1.2 plasmid (CloneJET PCR Cloning Kit, Thermo Fisher Scientific). .. Then, the loxP-dsRed-loxP fragment was cloned from pMSCV-loxP-dsRed-loxP-3xHA-Puro-WPRE (Addgene plasmid #32703). .. The loxP-dsRed-loxP fragment and the above modified pJet1.2 plasmid were digested with restriction enzymes AgeI (NEB, R0552S) and PacI (NEB, R0547S) and then ligated using T4 DNA ligase (NEB, M0202S) to get pAW63-Tex10 plasmid.

    Article Title: Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development
    Article Snippet: The PCR product from Gibson assembly was purified and cloned into pJet1.2 plasmid (CloneJET PCR Cloning Kit, Thermo Fisher Scientific). .. Then, the loxP-dsRed-loxP fragment was cloned from pMSCV-loxP-dsRed-loxP-3xHA-Puro-WPRE (Addgene plasmid #32703). .. The loxP-dsRed-loxP fragment and the above modified pJet1.2 plasmid were digested with restriction enzymes AgeI (NEB, R0552S) and PacI (NEB, R0547S) and then ligated using T4 DNA ligase (NEB, M0202S) to get pAW63-Tex10 plasmid.

    Article Title: Epithelial WNT secretion drives niche escape of developing gastric cancer
    Article Snippet: .. Cells were transfected using 30 μg of pMSCV-loxP-dsRed-loxP-eGFP-Puro-WPRE (Addgene, Plasmid #32,702) or pMSCV-loxP-dsRed-loxP-Cited2-3HA-Puro-WPRE (Addgene, Plasmid #32,703). .. Cold Cell Recovery Solution (Corning) was then added and incubated until the Matrigel dissolved.

    Article Title: Epithelial WNT secretion drives niche escape of developing gastric cancer.
    Article Snippet: The pellet was resuspended in 5 ml of ++ medium and plated in a 25 cm 2 cell culture flask (Corning). .. Cells were transfected using 30 μg of pMSCV-loxP-dsRed-loxP-eGFP-Puro-WPRE (Addgene, Plasmid #32702) or pMSCV-loxP-dsRed-loxP-Cited2-3HA-Puro-WPRE (Addgene, Plasmid #32703). .. DNA was mixed AR TIC LE IN PR ES S with Lipofectamine 2000 transfection reagent (ThermoFisher) to a total volume of 250 μl and incubated for 30 min at room temperature, following which 250 μl of Opti-MEM medium (ThermoFisher) was further added.

    other:

    Article Title: Lineage Tracing and Single-Cell RNA Sequencing Reveal a Common Transcriptional State in Breast Cancer Tumor–Initiating Cells Characterized by IFN/STAT1 Activity
    Article Snippet: To generate the dual color-switching LT reporter (EFS-loxP-dsRed-loxP-Nep), the loxP-dsRed-loxP-GFP sequence with attB1/B2 recombination sites was amplified from pMSCV-loxP-dsRed-loxP-eGFP-Puro-WPRE (a gift from Dr. Hans Clevers, Hubrecht Institute, Utrecht, Netherlands; Addgene plasmid #32702, RRID: Addgene_32702; ref. ) by two-step PCR and then incorporated into the pDonor233 vector with the P1/P2 compatible recombination sites by BP Gateway Cloning.

    Transfection:

    Article Title: Epithelial WNT secretion drives niche escape of developing gastric cancer
    Article Snippet: .. Cells were transfected using 30 μg of pMSCV-loxP-dsRed-loxP-eGFP-Puro-WPRE (Addgene, Plasmid #32,702) or pMSCV-loxP-dsRed-loxP-Cited2-3HA-Puro-WPRE (Addgene, Plasmid #32,703). .. Cold Cell Recovery Solution (Corning) was then added and incubated until the Matrigel dissolved.

    Article Title: Epithelial WNT secretion drives niche escape of developing gastric cancer.
    Article Snippet: The pellet was resuspended in 5 ml of ++ medium and plated in a 25 cm 2 cell culture flask (Corning). .. Cells were transfected using 30 μg of pMSCV-loxP-dsRed-loxP-eGFP-Puro-WPRE (Addgene, Plasmid #32702) or pMSCV-loxP-dsRed-loxP-Cited2-3HA-Puro-WPRE (Addgene, Plasmid #32703). .. DNA was mixed AR TIC LE IN PR ES S with Lipofectamine 2000 transfection reagent (ThermoFisher) to a total volume of 250 μl and incubated for 30 min at room temperature, following which 250 μl of Opti-MEM medium (ThermoFisher) was further added.



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